Effects and mechanism of carbonyl cyanide chlorophenylhydrazone (CCCP) on antimicrobial activity of florfenicol (FF) and thiamphenicol (TAP) were investigated against amphenicol-resistant Actinobacillus pleuropneumoniae and Pasteurella multocida isolated from diseased swine. Skin contact with cyanide salts can irritate and produce sores. Application: Carbonyl Cyanide m-Chlorophenylhydrazone is A proton ionophore commonly used as an uncoupling agent and inhibitor of photosynthesis. The synthesis of a mitochondria-targeted derivative of the classical mitochondrial uncoupler carbonyl cyanide-m-chlorophenylhydrazone (CCCP) by alkoxy substitution of CCCP with n-decyl(triphenyl)phosphonium cation yielded mitoCCCP, which was able to inhibit the uncoupling action of CCCP, tyrphostin A9 and niclosamide on rat liver mitochondria, but not that of 2,4 Activity of the efflux pump system was assessed using the efflux pump inhibitor, Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP). Conclusions: CO-induced vasoconstriction involves the generation of reactive oxygen species, which, when negated, allows for the expression of vasodilatory pathways which are masked by the primary oxidative stress response to this gas. CAS Number: 555-60-2. Ion leakage was expressed relative to the total amount of potassium released after addition of 1 M of the pore-forming lantibiotic nisin (100% efflux). Session: P030 Colistin resistance: detection, mechanisms and synergy Category:23 April 2017, 3d. Possible mechanisms of action of Nu-3. The growth-inhibitory effect of carbonyl cyanide m-chlorophenylhydrazone (CCCP) was less on members of the family Enterobacteriaceae (halotolerant organisms) than it was on species of Vibrio (moderately halophilic organisms). Download scientific diagram | Effects of carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (DNP) on selenite uptake by roots We propose a simple model that accounts for the ability of the weak acid FCCP (Carbonylcyanide-p-trifluoromethoxyphenylhydrazone) to both transport protons across phospholipid bilayer membranes and uncouple oxidation from phosphorylation in mitochondria. further research is required to fully characterize its molecular mechanism of action as an EP inhibitor. Molecular Weight: 204.62. National Center for Biotechnology Information. No Article Media. The antibacterial activity of these compounds is based on their protonophore action that has been demonstrated in artificial lipid bilayers , where both antibiotics were found to be more active than the very strong classic uncoupler of mitochondrial oxidative phosphorylation carbonyl cyanide-m-chlorophenyl hydrazone (CCCP). The kinetic mechanism by which CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across membranes. Kasianowicz J, Benz R, McLaughlin S. J Membr Biol, 82(2):179-190, 01 Jan 1984 Cited by: 61 articles | PMID: 6096547 Results: The MIC of ethidium bromide against S. aureus strains RN-4220 and IS-58 was reduced by association with -pinene. Policies. belongs to the class of organic compounds known as phenylhydrazines. Animal Research: Tumour Biol. Abstract. Five to six-month-old plants at the rosette stage were used in all experiments. When sodium chloride concentration increased from 0.5 to 0.85 M, this effect was more pronounced for Escherichia coli; it remained Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) 2014 Uncoupling of oxidative phosphorylation by carbonyl cyanide phenylhydrazones. Moreover, in cells pretreated with oligomycin plus carbonyl cyanide m-chlorophenylhydrazone and suspended in a nominally calcium-free medium, thapsigargin produces transient increases in cytosolic calcium identical to those in the control cells. [Google Scholar] Blum, J.J.; Sanadi, D.R. Phenylhydrazines are compounds containing a phenylhydrazide moiety, which consists of a hydrazide substituent attached to a phenyl group. Carbonyl cyanide m-chlorophenylhydrazone (CCCP), as a protonophore, in combination with antibiotics exhibited potentiating antibacterial activity.To improve CCCP's potency and toxicity, a series of aromatic hydrazones were synthesized and their antimicrobial activity was evaluated; amongst them, compounds 2e and 2j with a strong para-electron support this sequence of events for the PEP005 mechanism of action. Taken together, we concluded that Br-CA compound has a novel bactericidal mechanism and the potential to mitigate antibiotics resistance crisis. Displays several effects on cellular calcium. CCCP carbonyl cyanide m-chlorophenylhydrazone transmembrane potential EGTA ethylene glycol tetraacetic acid cells; however, its mechanism of action is not fully understood. carbonyl cyanide m-chlorophenylhydrazone (CCCP) (10mg/L), which decreased the MICs of Chlorhexidine and Medihex-4 by 2 128 fold but had no impact on the MICs of Benzalkonium chloride, Trigene and Mediscrub. Some characteristics of m-Cl-CCP action on mitochondria and chloroplasts. Carbonyl Cyanide m-Chlorophenylhydrazine (CCCP) Reverses Resistance to Colistin, but Not to Carbapenems and Tigecycline in Multidrug-Resistant Enterobacteriaceae. In particular, a lattice-cellular automaton was employed to simulate a detailed two-compartment model of a two-dimensional brain-cell system. the addition of 5 M CCCP (carbonyl cyanide m-chlorophenylhydrazone; (C). Hence, the quest to find other agents that will synergistically restore the of the mechanism of action of the aryloxyphenoxypropionate and cyclohexanedione herbicides. In bullfrog sympathetic ganglia, the ryanodine-sensitive Ca2+ store and mitochondria modulate [Ca2+] within nerve terminals. It is a nitrile, hydrazone and protonophore. National Institutes of Health. Four parameters characterize this model: the adsorption coefficients of the anionic and neutral forms of the weak acid onto the interface ( A and HA) and the rate constants for the movement of While it is widely recognized that PPi, inorganic pyrophosphate; CCCP, carbonyl cyanide m-chlorophenylhydrazone; PCMBS, p-chloromercuribenzenesulphonic acid. Carbonyl Cyanide m-Chlorophenylhydrazone is a protonophore (H ionophore) and an uncoupler of oxidative phosphorylation in mitochondria. Abstract. Department of Health and Human Services. The effect of carbonyl-cyanide m-chlorophenyl-hydrazone (CCCP) on intracytoplasmic membrane (ICM) assembly was examined in the purple bacterium Rhodobacter sphaeroides. Transport activities were best under slightly acidic conditions and were inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazone, demonstrating an H +-coupled transport mechanism. No Supplementary Data. The electrons for hydrogen photoevolution are mainly from the photolysis of water (90%). The facilitated transport of protons across the biological membrane by anionic protonophore is achieved as follows. 2008). As a positive control for DiBAC 4 (3) and ACMA, CCCP (carbonyl cyanide 3-chlorophenylhydrazone; Sigma-Aldrich C2759), a protonophore which uncouples PMF, was dissolved in DMSO and added to incubating liquid cultures at least 5 min before imaging, to a final concentration of 1 mM. Many ionophores serve as research tools to study ion transport and alter physiological processes in cells. Carbonyl Cyanide m-Chlorophenyl hydrazone (CCCP) is a hydrogen ionophore, which causes uncoupling of the proton gradient during oxidative phosphorylation. Research so far indicates that it acts on cell membrane [3, 4] and also inhibits the synthesis of like carbonyl cyanide m-chlorophenylhydrazone [8]. (L96, L97) | Organic nitriles are converted into cyanide ions through the action of cytochrome P450 enzymes in the liver. Ionomycin is a calcium ionophore commonly used to study calcium flux in cells. Antimicrobial peptides. CCCP is a protonophore which is a widely used uncoupler of mitochondrial oxidative phosphorylation. p-Coumaric acid did not affect the integrity of cell wall and plasmatic membrane and neither produced oxidative stress. Carbonyl Cyanide m-Chlorophenylhydrazine (CCCP) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant enterobacteriaceae. Front Microbiol. 2017;8:228. doi:10.3389/fmicb.2017.00228 J. Abbreviations: CCCP, carbonyl cyanide m-chlorophenylhydrazone; CFU, colony forming unit; LEV, levofloxacin; MIC, minimum inhibitory concentration. Experiments on chloroplast suspensions showed that decreased external pH also induced an analogous response and that its magnitude depended on the magnitude of pH The importance of this mechanism of resistance in clinical settings is reflected in the increasing number of reports of multidrug resistant isolates. Vascular tissues generate carbon monoxide (CO) via heme oxygenase (HO)-dependent and HO-independent pathways. The mechanisms of action of tea tree oil and three of its components, 1,8-cineole, terpinen-4-ol, and -terpineol, against Staphylococcus aureus ATCC 9144 were investigated. The possible mechanisms of its action may involve the regulation of drugmetabolizing genes. Sigma-Aldrich. Here, we characterized the effects of the typical mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) on vascular function in rat mesenteric arteries and aorta and elucidated the potential mechanisms. Carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, leads to AMPK activation and Parkin-dependent mitophagy, respectively. However, the detailed biochemical mechanism of how CCCP induces autophagy or mitophagy has not been investigated yet. The accumulation of KB-5246 in a quinolone-susceptible strain of Staphylococcus aureus was about 70 times that of norfloxacin. The kinetic mechanism by which CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across membranes. Broth microdilution and time-kill assays indicated that CCCP dose-dependently Iodoacetate (5 raM) inhibits PTS [2] but has no effect on D-glucose dehydrogenase, p-Chloromercuri- benzoate and carbonyl cyanide m-chlorophenylhydrazene inhibit both PTS and glucose oxidation. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was used as a positive control. To assess the contribution of the efflux pump to TonB-dependent macrolide efflux, MIC levels were determined using broth microdilution assay in the absence or presence of 3 mg/L carbonyl cyanide m-chlorophenylhydrazone (CCCP), 50 mg/L phenylalanine-arginine -naphthylamide (PAN) or 10 mg/L sodium orthovanadate (SOV). However, the exact molecular mechanisms of toxic action of Zn 2+ and selenite, namely, role of mitochondria and reactive oxygen species Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), oligomycin, valinomycin, bongkrekic acid, ZnCl 2, and Na 2 SeO 3 were purchased from Sigma (St. Louis, MO). Disrupting membrane potential confers protection against daptomycin and LL-37. Carbonyl cyanide m-chlorophenyl hydrazone (T3D1697) Carbonyl cyanide m-chlorophenyl hydrazone is a chemical compound of cyanide. To verify the integrity of the mitochondrial inner membrane, the respiratory control rate and the uncoupling activity of carbonyl cyanide m-chlorophenylhydrazone (CCCP) were determined for each mitochondrial preparation; these values ranged between 2.5 and 2.7 , which are normal values for yeast mitochondria . In the mechanism of action of carbonyl cyanide m-chlorophenylhydrazone the key step may be the electrostatic interaction of its protonated form and DOI: 10.1016/0005-2728(74)90134-0 Corpus ID: 13539506; Effects of carbonyl cyanide m-chlorophenylhydrazone and hydroxylamine on the photosystem II electron exchange mechanism in 3-(3,4-dichlorophenyl)-1,1-dimethylurea treated algae and chloroplasts. From: Methods in Enzymology, 2014 Download as PDF About this page A Biblioteca Virtual em Sade uma colecao de fontes de informacao cientfica e tcnica em sade organizada e armazenada em formato eletrnico nos pases da Regio Latino-Americana e do Caribe, acessveis de forma universal na Internet de Modes of action for antimicrobials vary depending on their biochemical properties. No Citations. A7506. Sigma-Aldrich. the physiological mitochondrial K ATP channel opener GTP was therefore used to investigate the action of the two sulfonylureas. HHS Vulnerability Disclosure. FOIA. We demonstrated that a significant volume of H2 gas could be photobiologically produced by a marine green alga Platymonas subcordiformis when an uncoupler of photophosphorylation, carbonyl cyanide mchlorophenylhydrazone (CCCP), was added after 32 h of anaerobic dark incubation, whereas a negligible volume of H2 gas was produced without The addition of pro Finally, it was shown that the compound produced an increase in oxygen consumption. The plasma membrane H+-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. 14 In this regard, antimicrobial peptides (AMPs) have attracted attention as a potential candidate B acterial pathogens have developed resistance to most of the conventional antibiotics available today. 8600 Rockville Pike, Bethesda, MD, 20894 USA. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) ([ (3-chlorophenyl)hydrazono]malononitrile) is a chemical inhibitor of oxidative phosphorylation. It is a nitrile, hydrazone and protonophore. In general, CCCP causes the gradual destruction of living cells and death of the organism. The uncoupling effect of CCCP accelerates electron transfer from water due to a disruption of the proton motive force and release of pH across the thylakoid membrane and thus enhances the accessibility of electron and H + to hydrogenase. The availability of compounds which inhibit a given route of D-glucose entry could be interesting in some experiments. A new mechanism of action for the anticancer drug Mitomycin C: Mechanism-based inhibition of thioredoxin reductase. Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) Published 2014 View Full Article Home; Publications; Publication Search; Publication Details; Title Mechanisms and physiological role of polarity in plants Kasianowicz J, Benz R, McLaughlin S. J Membr Biol, 82(2):179-190, 01 Jan 1984 Cited by: 63 articles | PMID: 6096547 Na +, on the other hand, had no effect on sucrose transport. Multidrug resistance (MDR) to antibiotics presents a serious therapeutic problem in the treatment of bacterial infections. carbonyl cyanide m-chlorophenylhydrazone (2 M; CCCP; Sigma) in glucose-free medium for 1 hr. Uncouplers of oxidative phosphorylation such as cyanide carbonyl m-chlorophenylhydrazone (CCCP) foster proton (H+) permeability of bacterial and other membranes; counter cation movement is required for the effect of the uncoupler on Carbonyl cyanide m-chlorophenylhydrazone Carbonyl cyanide m-chlorophenylhydrazone increased the accumulation of norfloxacin about eightfold, but it did not influence that of KB-5246. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was used as a positive control. Carbonyl Cyanide Chlorophenylhydrazone CCCP/FCCP is a lipid-soluble weak acid and a potent mitochondrial uncoupling agent that increases the proton permeability across the mitochondrial inner membranes, thus dissipating the transmembrane potential and depolarizing the mitochondria. The present study extends the previous research by using a novel numerical scheme for the analysis of potassium buffering mechanisms in the extracellular brain-cell microenvironment. The mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone (1 mol/L), used as a positive control to of the lack of a plausible mechanism for the University Group Diabetes Program study results. Daptomycin mechanism of action is complex and not entirely understood. Four parameters are required to characterize this model: the rate constant for the movement of A- across the The antibacterial activity of these compounds is based on their protonophore action that has been demonstrated in artificial lipid bilayers , where both antibiotics were found to be more active than the very strong classic uncoupler of mitochondrial oxidative phosphorylation carbonyl cyanide-m-chlorophenyl hydrazone (CCCP). Treatment of nonenergized L. monocytogenes with 5 mM eugenol, 40 mM Cinnamaldehyde, or 10 M carbonyl cyanide m-chlorophenylhydrazone (OXA), two chemotherapeutic agents used in CRC treatment. Trimethoprim, 98% (HPLC) Pricing. Wells containing only culture medium and bacterial inoculums were used as negative control. 2014 Jun;35(6):5717-22. However, the detailed biochemical mechanism of how CCCP induces autophagy or Plants were sprayed with 1kg a.i./ha Pq as a 1 % (v/v) solution (5x10-4 molL-1) of Gramoxone. Background and Purpose. Aminoglycosides are broad-spectrum antibiotics whose mechanism of action is under debate. The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 32:7658-7668 mechanism of the fluorescence decline induced by strong actinic Nanba M and Katoh S (1986) The site and mechanism of duroquinol light in PS II particles under different redox conditions. CCCP blocks generation of the electrochemical proton gradient required for integral membrane protein insertion. PEP005 and carbonyl cyanide m-chlorophenylhydrazone were dissolved in 100% acetone and diluted in complete medium; the acetone concentration in the medium did not exceed 1%. The quantal acetylcholine release and the ultrastructural effects of the metabolic inhibitor carbonyl cyanide m-chlorophenylhydrazone have been examin T7883. We used caffeine (10mM) a Background: Carbapenems (CAR), colistin (CST), and tigecycline (TGC) alone or in combination therapy has become the last-resort antibiotics for treating infections caused by multidrug resistant (MDR) bacteria. In addition, the energy decoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP), which is used for in-vitro testing of the expression of bacterial efflux pumps because it is highly toxic to eukaryotic cells 9 and proton pump inhibitors, 10 was also discovered. Kasianowicz J, Benz R, McLaughlin S Author information ORCIDs linked to this article Benz R, 0000-0002-9510-9265, Jacobs University Bremen GmbH The Journal of Membrane Biology , 01 Jan 1984, 82 (2): 179-190 RTDs were added at 5 and 10 MIC; controls were incubated without peptide. The mechanism of action of this compound was analysed using fluorescent probes. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) ([(3-chlorophenyl)hydrazono]malononitrile) is a chemical inhibitor of oxidative phosphorylation. It is a nitrile , hydrazone and protonophore . In general, CCCP causes the gradual destruction of living cells and death of the organism. J Membr Biol 82:179190 (1984) However, resistance to these reserve antibiotics are increasingly being reported worldwide. Heytler, P.G. Carbonyl cyanide m-chlorophenyl hydrazone ( CCCP; also known as [ (3-chlorophenyl)hydrazono]malononitrile) is a chemical inhibitor of oxidative phosphorylation. L-Ascorbic acid, reagent grade, crystalline. We collect cookies for vital website function and to better serve our customers. I. CCCP/FCCP is a lipid-soluble weak acid and a potent mitochondrial uncoupling agent that increases the proton permeability across the mitochondrial inner membrane, thus dissipating the transmembrane potential and depolarizing the mitochondria. Contact. Many cancer chemotherapeutic agents (Grad 2001) including Tam (Dietze 2001) exert their anti-cancer The activities of extracts, fractions, and compounds were improved in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP) as an efflux pump inhibitor to as much as >128 folds. National Library of Medicine. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p The kinetic mechanism by which CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across membranes. The actual mechanism of action is not yet fully understood but has been proposed variously to involve cell lysis, breakdown of (NPN), carbonyl cyanide-m-chlorophenylhydrazone, and Re LPS from Salmo-nella enterica serovar Minnesota R595 (Re mutant) were purchased from Sigma Carbonyl cyanide m-chlorophenylhydrazone PIMT induction seems to be specifically associated with CCCP mechanisms of action since treatments with rotenone, a complex I inhibitor, did not cause an induction of PIMT in mitochondrial fraction. Carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, leads to AMPK activation and Parkin-dependent mitophagy, respectively. (illumination 130 E m-2s-1, 16h light period, 22-25 C) for 3-4 months in soil containers and then transferred to field conditions. The kinetic mechanism by which CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across membranes. Alternate Names: (3-Chlorophenyl)hydrazonomalononitrile; Mesoxalonitrile 3-chlorophenylhydrazone; CCCP. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was added at 20 M for 10 minutes prior to daptomycin (200 g/mL daptomycin, A) or LL-37 (2.5 g/mL, B) challenge. Carbonyl cyanide 3-chlorophenylhydrazone, 97% (TLC), powder. Purity: 97%. CCCP disrupts ATP synthesis by transporting protons across the mitochondrial inner membrane, interfering with the proton gradient. emrB is a translocase in the emrB -TolC efflux protein in E. coli. It recognizes substrates including carbonyl cyanide m-chlorophenylhydrazone (CCCP), nalidixic acid, and thioloactomycin. Six isolates of K. pneumoniae were chosen for Chronic inhalation of cyanide causes breathing difficulties, chest pain, vomiting, blood changes, headaches, and enlargement of the thyroid gland. tion of H+ inux using the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) induced a photosyn-thetic response that was similar with a VP-induced response. Potassium cyanide (3 or 5 mmol/L), carbonyl cyanide m-chlorophenylhydrazone (CCCLP; 5 or 10 pmol/L from a concentrated stock solution in ethanol), tert- butyl hydroperoxide (TBHP; 0.1, 0.25 or 0.5 mmol/L) and the calcium ionophores A23187 (10 to 50 pmol/L from concen- trated stock in ethanol) and ionomycin (5, 10 and 15 pmoVL We investigated the bactericidal mode of action of the anti-tubercular F 1 F o by increasing the rate of metabolic reactions in central carbon metabolism that produce ATP by an alternative mechanism, Mutants of Mycobacterium smegmatis unable to grow at acidic pH in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone. Of note, DAP was also found to induce a group of genes that was previously associated with exposure to carbonyl cyanide m-chlorophenylhydrazone, a proton ionophore, reflecting its ability to disrupt the membrane and induce ion leakage (Muthaiyan et al. Therefore, generating novel medicinal antibiotics as an alternative to control and treat the infections caused by these pathogens is essential. In the mechanism of action of carbonyl cyanide m-chlorophenylhydrazone the key step may be the electrostatic interaction of its protonated form and one of the forms of activated oxygen at the catalytic centre of cytochrome P-450. At concent Pricing. (Amp) and ciprofloxacin (Cip) were induced. To investigate the role of interference with energy generation in the mechanism of action, the cellular and extracellular ATP levels of cells in HEPES buffer at 20 degrees C were measured. Bacterial Strains E-cadherin is shown here to be a cellular substrate for a bacterial toxin and represents the identification of a mechanism of action, cell-surface proteolytic activity, for a bacterial toxin. Carbonyl cyanide m-chlorophenylhydrazone (CCCP), phosphate buffer, and Sodium chloride were purchased from sigma Aldrich (Missouri, United States), GE Healthcare Life Sciences China (Beijing, China), and Shanghai Runjie Chemical Reagent Co., Ltd (Shanghai, China), respectively. The inhibitory effect of carbonyl cyanide m-chlorophenylhydrazone (CCCP) on the electron transport system of chloroplasts was investigated. Proton ionophores such as carbonyl cyanide m-chlorophenylhydrazone (CCCP) could destroy membrane potential by eliminating the proton gradient, thus it was used as a positive control in the study. In Salmonella enterica, the most common etiological agent of food borne salmonellosis worldwide, MDR is The effects and mechanisms of chemical mitochondrial uncouplers on vascular function have never been identified. ICM formation was induced for 8h, followed by a 4-h exposure to CCCP. No References. Biochemistry 1963, 2, 357361. Thus, beside We demonstrate that a simple kinetic model describes the transport of protons across lipid bilayer membranes by the weak acid CCCP (carbonyl cyanidem-chlorophenylhydrazone). Activation of myosin adenosine triphosphatase by carbonyl cyanide p-chlorophenylhydrazone. Mechanism of action.
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